Aurora B-dependent Ndc80 degradation regulates kinetochore composition in meiosis.
| Publication Type | Academic Article |
| Authors | Chen J, Liao A, Powers E, Liao H, Kohlstaedt L, Evans R, Holly R, Kim J, Jovanovic M, Ünal E |
| Journal | Genes Dev |
| Volume | 34 |
| Issue | 3-4 |
| Pagination | 209-225 |
| Date Published | 01/09/2020 |
| ISSN | 1549-5477 |
| Keywords | Aurora Kinase B, Kinetochores, Meiosis, Nuclear Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins |
| Abstract | The kinetochore complex is a conserved machinery that connects chromosomes to spindle microtubules. During meiosis, the kinetochore is restructured to accommodate a specialized chromosome segregation pattern. In budding yeast, meiotic kinetochore remodeling is mediated by the temporal changes in the abundance of a single subunit called Ndc80. We previously described the regulatory events that control the timely synthesis of Ndc80. Here, we report that Ndc80 turnover is also tightly regulated in meiosis: Ndc80 degradation is active in meiotic prophase, but not in metaphase I. Ndc80 degradation depends on the ubiquitin ligase APCAma1 and is mediated by the proteasome. Importantly, Aurora B-dependent Ndc80 phosphorylation, a mark that has been previously implicated in correcting erroneous microtubule-kinetochore attachments, is essential for Ndc80 degradation in a microtubule-independent manner. The N terminus of Ndc80, including a 27-residue sequence and Aurora B phosphorylation sites, is both necessary and sufficient for kinetochore protein degradation. Finally, defects in Ndc80 turnover predispose meiotic cells to chromosome mis-segregation. Our study elucidates the mechanism by which meiotic cells modulate their kinetochore composition through regulated Ndc80 degradation, and demonstrates that Aurora B-dependent regulation of kinetochores extends beyond altering microtubule attachments. |
| DOI | 10.1101/gad.333997.119 |
| PubMed ID | 31919192 |
| PubMed Central ID | PMC7000919 |