A Combination of Two Human Monoclonal Antibodies Prevents Zika Virus Escape Mutations in Non-human Primates.
| Publication Type | Academic Article |
| Authors | Keeffe J, Van Rompay K, Olsen P, Wang Q, Gazumyan A, Azzopardi S, Schaefer-Babajew D, Lee Y, Stuart J, Singapuri A, Watanabe J, Usachenko J, Ardeshir A, Saeed M, Agudelo M, Eisenreich T, Bournazos S, Oliveira T, Rice C, Coffey L, MacDonald M, Bjorkman P, Nussenzweig M, Robbiani D |
| Journal | Cell Rep |
| Volume | 25 |
| Issue | 6 |
| Pagination | 1385-1394.e7 |
| Date Published | 11/06/2018 |
| ISSN | 2211-1247 |
| Keywords | Antibodies, Monoclonal, Mutation, Zika Virus |
| Abstract | Zika virus (ZIKV) causes severe neurologic complications and fetal aberrations. Vaccine development is hindered by potential safety concerns due to antibody cross-reactivity with dengue virus and the possibility of disease enhancement. In contrast, passive administration of anti-ZIKV antibodies engineered to prevent enhancement may be safe and effective. Here, we report on human monoclonal antibody Z021, a potent neutralizer that recognizes an epitope on the lateral ridge of the envelope domain III (EDIII) of ZIKV and is protective against ZIKV in mice. When administered to macaques undergoing a high-dose ZIKV challenge, a single anti-EDIII antibody selected for resistant variants. Co-administration of two antibodies, Z004 and Z021, which target distinct sites on EDIII, was associated with a delay and a 3- to 4-log decrease in peak viremia. Moreover, the combination of these antibodies engineered to avoid enhancement prevented viral escape due to mutation in macaques, a natural host for ZIKV. |
| DOI | 10.1016/j.celrep.2018.10.031 |
| PubMed ID | 30403995 |
| PubMed Central ID | PMC6268006 |