Forward genetic screen of human transposase genomic rearrangements.

Publication Type	Academic Article
Authors	Henssen A, Jiang E, Zhuang J, Pinello L, Socci N, Koche R, Gonen M, Villasante C, Armstrong S, Bauer D, Weng Z, Kentsis A
Journal	BMC Genomics
Volume	17
Pagination	548
Date Published	08/04/2016
ISSN	1471-2164
Keywords	Gene Rearrangement, Genetic Testing, Genome, Human, Genomics, Transposases
Abstract	BACKGROUND: Numerous human genes encode potentially active DNA transposases or recombinases, but our understanding of their functions remains limited due to shortage of methods to profile their activities on endogenous genomic substrates. RESULTS: To enable functional analysis of human transposase-derived genes, we combined forward chemical genetic hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) screening with massively parallel paired-end DNA sequencing and structural variant genome assembly and analysis. Here, we report the HPRT1 mutational spectrum induced by the human transposase PGBD5, including PGBD5-specific signal sequences (PSS) that serve as potential genomic rearrangement substrates. CONCLUSIONS: The discovered PSS motifs and high-throughput forward chemical genomic screening approach should prove useful for the elucidation of endogenous genome remodeling activities of PGBD5 and other domesticated human DNA transposases and recombinases.
DOI	10.1186/s12864-016-2877-x
PubMed ID	27491780
PubMed Central ID	PMC4973553