High-sensitivity flow cytometric analysis of mast cell clustering in systemic mastocytosis: a quantitative and statistical analysis.
| Publication Type | Academic Article |
| Authors | Pozdnyakova O, Laplante C, Li B, Rosner B, Dorfman D |
| Journal | Leuk Lymphoma |
| Volume | 56 |
| Issue | 6 |
| Pagination | 1735-41 |
| Date Published | 11/03/2014 |
| ISSN | 1029-2403 |
| Keywords | Flow Cytometry, Mast Cells, Mastocytosis, Systemic, Proto-Oncogene Proteins c-kit |
| Abstract | We characterized recently identified event clusters in systemic mastocytosis (SM) by calculating the coefficient of variation (CV, %) of CD117 and side scatter (SSC), based on the mean fluorescence intensity of mast cells using flow cytometry. Seventy-five samples were from patients with SM and 124 samples from patients negative for SM (non-SM). Discrete cluster formation seen in 50 cases correlated with significantly lower CV for SSC (46.1% vs. 61.0%, p < 0.0001) and CD117 (64.5% vs. 80.5%, p < 0.0001) for SM vs. non-SM samples. A combined CVCD117 + SSC of < 125 showed a sensitivity of 80% and specificity of 80% for SM with PPV of 67% and NPV of 80%. Probability scores of having SM, generated based on CVs for SSC and CD117, were significantly higher in patients with SM than non-SM (0.55 vs. 0.17, respectively; p < 0.001). Flow cytometric-based quantitative analysis of event clustering is a useful approach for diagnosing and monitoring patients with SM. |
| DOI | 10.3109/10428194.2014.970546 |
| PubMed ID | 25284492 |