A Robust Method for the Purification and Characterization of Recombinant Human Histone H1 Variants.
| Publication Type | Academic Article |
| Authors | Osunsade A, Prescott N, Hebert J, Ray D, Jmeian Y, Lorenz I, David Y |
| Journal | Biochemistry |
| Volume | 58 |
| Issue | 3 |
| Pagination | 171-176 |
| Date Published | 01/08/2019 |
| ISSN | 1520-4995 |
| Keywords | Histones, Protein Engineering, Recombinant Proteins |
| Abstract | Higher order compaction of the eukaryotic genome is key to the regulation of all DNA-templated processes, including transcription. This tightly controlled process involves the formation of mononucleosomes, the fundamental unit of chromatin, packaged into higher order architectures in an H1 linker histone-dependent process. While much work has been done to delineate the precise mechanism of this event in vitro and in vivo, major gaps still exist, primarily due to a lack of molecular tools. Specifically, there has never been a successful purification and biochemical characterization of all human H1 variants. Here we present a robust method to purify H1 and illustrate its utility in the purification of all somatic variants and one germline variant. In addition, we performed a first ever side-by-side biochemical comparison, which revealed a gradient of nucleosome binding affinities and compaction capabilities. These data provide new insight into H1 redundancy and lay the groundwork for the mechanistic investigation of disease-driving mutations. |
| DOI | 10.1021/acs.biochem.8b01060 |
| PubMed ID | 30585724 |
| PubMed Central ID | PMC6541009 |